Human Reticulocalbin 3 (RCN3) ELISA Kit

Cat.#:

JL49092

Regulatory Status:

RUO

Alternative:

RLP49; EF-Hand Calcium Binding Domain; EF-hand calcium-binding protein RLP49

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.312-20ng/mL

Sensitivity:

0.12ng/mL

Specificity:

This kit recognizes Human RCN3 in samples. No significant cross-reactivity or interference between Human RCN3 and analogues was observed.

Sample Type:

tissue homogenates;cell lysates and other biological fluids

Assay Procedure:

Research Area:

Metabolism

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| RCN3

Reticulocalbin 3 (RCN3) enables calcium ion binding activity. Involved in several processes, including collagen biosynthetic process; positive regulation of peptidase activity; and regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction. Located in endoplasmic reticulum.RCN3 (Reticulocalbin 3) is a Protein Coding gene. Diseases associated with RCN3 include Myopathy, Distal, 3 and Neonatal Respiratory Failure. Gene Ontology (GO) annotations related to this gene include calcium ion binding. An important paralog of this gene is RCN1.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	20	10	5	2.5	1.25	0.625	0.312	0
OD	2.22	1.56	0.92	0.63	0.42	0.25	0.18	0.08
Corrected OD	2.14	1.48	0.84	0.55	0.34	0.17	0.1	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human RCN3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-101	96
Cell Lysates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Human RCN3 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	88-96	90-110
1 : 2	Average (%)	93	96
1 : 4	Range (%)	87-108	105-115
1 : 4	Average (%)	98	108